PdtDtShtProduct Data SheetProduct Name:CAS No.: Cat. No.:MWt:Formula:Purity :P 220771247819-59-5HY-13865315.32C12H7F2NO3S2>98%Solubility:DMSO 63 mg/ml; Water <1 mg/mlMechanisms: Pathways:Cell Cycle/DNA Damage; Target:Deubiquitinase Biological Activity:()()P22077 is a potent inhibitor of ubiquitin-specific protease (USP) 7 (EC50 = 8.6 uM), P22077 alsoinhibits the closely related deubiquitinase (DUB) USP47. IC50 Value: 8.6 uM (EC50) [1] Target: USP7; USP47 in vitro: P22077 potently induces apoptosis in NB cells with an intact USP7-HDM2-p53 axis but notin NB cells with mutant p53 or without human homolog of MDM2 (HDM2) expression. P22077stabilized p53 by inducing HDM2 protein degradation in NB cells. P22077 also significantlyaugmented the cytotoxic effects of doxorubicin (Dox) and etoposide (VP-16) in NB cells with anintact USP7-HDM2-p53 axis. Moreover, P22077 was found to be able to sensitize chemoresistantLAN6NBllthLA-N-6 NB cells to chemotherapy [2].th[2]in vivo: In an in vivo orthotopic NB mouse model, P22077 significantly inhibited the xenograft growthof three NB cell lines [2]. ...References:[1]. Altun M, et al. Activity-based chemical proteomics accelerates inhibitor development fordeubiquitylating enzymes. Chem Biol. 2011 Nov 23;18(11):1401-12. [2]FanYHetalUSP7inhibitorP22077inhibitsneuroblastomagrowthviainducingp53mediated[2]. Fan YH, et al. USP7 inhibitor P22077 inhibits neuroblastoma growth via inducing p53-mediatedapoptosis. Cell Death Dis. 2013 Oct 17;4:e867. Caution: Not fully tested. For research purposes onlyMedchemexpress LLCwww.medchemexpress.com 18 Wilkinson Way, Princeton, NJ 08540,USAEmail: info@medchemexpress.com Web: www.medchemexpress.com